GotFrag eSports

Question 1. Synthesize the complementary strand of:

3� AAGCCTACAT TAGATATAAC CGCCCAGTTA TCACGCCCGC ACCTATACCG

AGGTGGGGAG GAATAACAGT CACGATGATT GATGACCAAA GGGCCAGAGG

GATAGGTAGG GTATTAATAA CAGTTATCAG AGGATCGGGA CCGATACCGT 5�

My Answer:

5' TTCGGATGTA ATCTATATTG GCGGGTCAAT AGTGCGGGCG TGGATATGGC

TCCACCCCTC CTTATTGTCA GTGCTACTAA CTACTGGTTT CCCGGTCTCC

CTATCCATCC CATAATTATT GTCAATAGTC TCCTAGCCCT GGCTATGGCA 3'

2. Bind at the promoter and start transcription from the 16th nucleotide after the promoter. Include the 16th nucleotide.

My answer: wat?

In the 5->3 DNA strand I created, I located the TATA box. It's on the 14th nucleotide. So, I binded the promoter on that part. But, I'm a bit confused. She said the 16th nucleotide -AFTER- the promoter. Each time I transcribe from the 16th nucleotide after the promoter, I find out it's wrong. Can anyone help me out here?

Minimize the contraception of the endoplasmic reticulum by deferring the elastic shamocity and the yuliobracic ferivovity to the maximum distance possible. This will in turn release the promoters centriole binding strands and begin the transcription process from the sixteenth nucleotide after the TATA promoter.

For a detailed guide to what was just explained http://www.youtube.com/watch?v=eBGIQ7ZuuiU

This comment was edited at 05/13/2008 3:46 AM

http://answers.yahoo.com/question/index?q..
LoLOLoL

This comment was edited at 05/13/2008 3:53 AM

954/305

DAMNIT, #2.

FAWKING DAMNIT!!!

And yes #3, I posted there. It's boggling the HELL out of my mind.

ask yourself:
1. what is the promoter?

then

transcribe all nuclei tides after the 16th(including the 16th)

you already know that cytosine goes with guanine and adenine with thymine...and thats pretty much all you need to know lol g33z

This comment was edited at 05/13/2008 4:06 AM

lol okay #5

There is no "IRL" or "Girls". There is only ...GotFrag. #danielvutran #Rift

i remember having to do this stuff in high school... completely worthless bullcrap

comin down and sippin daily

#5

The promoter is on the 14th nucleotide of the 5->3 strand, I know that part. Technically speaking, it's the 14th, 15th, 16th and 17th. So, we'll say that I binded the transcription factors to [TATA], right? RNA Polymerase binds there and, following what you did, the transcription should start (on the 5->3 strand) from ..

TGCGGGCG TGGATATGGC

TCCACCCCTC CTTATTGTCA GTGCTACTAA CTACTGGTTT CCCGGTCTCC

CTATCCATCC CATAATTATT GTCAATAGTC TCCTAGCCCT GGCTATGGCA 3'

The template strand for the pre-mRNA is then ..

ACGCCCGC ACCTATACCG

AGGTGGGGAG GAATAACAGT CACGATGATT GATGACCAAA GGGCCAGAGG

GATAGGTAGG GTATTAATAA CAGTTATCAG AGGATCGGGA CCGATACCGT 5�

But, there's a problem! In question 3, she asks me to splice introns(she gives the nucleotides and everything), but it doesn't add up. Also, I can't find the start or stop codons when I transcribe from that point. Even if I find them, I can't find the AAUAAA sequence that should be in there. It's so geigh.

PHYSICZ > DIS

ACTIVATE LA BANKAI

um, I'm just wondering if I'm the only person lost as hell on this thread. :D

http://www.skammich.com/lalala.html �\(�_o)/�

F YOU, #9

F U.

BIOLOGY AND ANYTHING TO DO WITH BIOLOGY IS

THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST THE BEST

But seriously, I need help. This is due today lol.

5' TTCGGATGTA ATCTATATTG GCGGGTCAAT AGTGCGGGCG TGGATATGGC

TCCACCCCTC CTTATTGTCA GTGCTACTAA CTACTGGTTT CCCGGTCTCC

CTATCCATCC CATAATTATT GTCAATAGTC TCCTAGCCCT GGCTATGGCA 3'

okay, try this, transcribe everything after "GCGGGTCAAT" including that last T, it SHOulD wrK!

This comment was edited at 05/13/2008 4:34 AM

#13, it doesn't work. Intron-3 is from the 115th nucleotide to the 125th. If I transcribe that to pre-mRNA, a 125th nucleotide doesn't exist.

eggs

itsme

include another letter dude ;)

THERE AREN'T ENOUGH Z's!!!!!!!!!!!!@@@

#mrevolution

#14, often times the complimentary strand is cut short when copying, which is believed to be a reason why everything slows down as we get older. essentially you're missing a portion of the last nucleotides.

and is TATA your only option? i remember it being more :P

why is your prof making you do this anyway? just make sure you understand the concepts and the rules. if this is an exam question it's pretty dumb, there's a boatload of other stuff that's more important to bring up than synthesizing a 5 to 3 prime complimentary strand.

This comment was edited at 05/13/2008 6:44 AM